FauND I (Nde I)

Concentration
10u/μl 
5 ' . . . C A↓T A T G . . . 3 '
3 ' . . . G T A T↑A C . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition
10u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65˚C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay
After 10-fold overdigestion with FauND I, 90% of the DNA fragments can be ligated and recut..

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of FauND I for 16 hours at 37˚C.

Ordering Information

Downloads
Manual
FauND I (Nde I)