Bgl II

Concentration
10u/μl 
5 ' . . . A↓G AT C T. . . 3 '
3 ' . . . T C TA G↑A . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 1mM DTT, 200μg/ml BSA and 50% glycerol.

Unit Definition
10u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
None.

Quality Control Assays
Ligation/ Recutting Assay
After 10-fold overdigestion with Bgl II, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Bgl II for 16 hours at 37˚C.

Ordering Information

Downloads
Manual
Bgl II