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ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit

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产品名称: ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit
产品型号: QV2010
产品展商: Vivantis Technologies Sdn. Bhd.
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简单介绍

Home ViPrimePLUS Real-Time PCR Kits Veterinary Infection qPCR/RT-qPCR Kits Canine & Feline ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit Description The ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Feline Leukaemia Virus genome in blood and bodily fluid samples.


ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit  的详细介绍
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ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit

Description
The ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Feline Leukaemia Virus genome in blood and bodily fluid samples.
Feline Leukaemia Virus (FeLV) is an oncogenic retrovirus that infects cats and causes leukaemia. It is often found in cats positive for feline immunodeficiency virus (FIV). There are 4 subgroups of FeLV; 1) Subgroup A which is found in naturally infected cats and tends to be less pathogenic, 2) Subgroup B which increases neoplastic development frequency, 3) Subgroup C which accounts for 1% of the infected cats, causes severe anemia and 4) Subgroup T which leads to lymphoid depletion and immunodeficiency. It is believed that subgroups C and D arise as the mutated form of subgroup A. The virus may be transmitted vertically (in utero or by milk) or horizontally (by secretions and excretions). Initial infection may be limited to lymphoid tissue but as second stage progresses, the virus may be circulated to the bone marrow, oesophagus, bladder, respiratory tract and salivary glands. Once infection reaches the bone marrow, mortality rates can raise up to 50-80%. The disorders due to the FeLV infections include immunosuppression, lymphoid tumour, anemia, reproduction problems and enteritis. 
ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit is intended for research use only.

Kit Component

  • FeLV Primers and Probe Mix (FeLV PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit RNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Feline samples (e.g. blood, body fluid)
Gene Target U3 region LTR
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

Ordering Information

Catalog No Description Pack Size
QV2010 ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit 150 reactions

Download
Manual

ViPrimePLUS Feline Leukaemia Virus RT-qPCR Kit

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