Concentration 3u/μl 5 ' . . . A G↓C T. . . 3 ' 3 ' . . . T C↑G A . . . 5 '
Storage Buffer 10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.
Unit Definition 3u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μ l or 50μl of assay buffer.
Thermal Inactivation 65˚C for 20 minutes.
Quality Control Assays Ligation/ Recutting Assay After 3-fold overdigestion with Alu I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 6u o f Alu I for 16 hours at 37˚C.
Ordering Information
Downloads Manual Alu I
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