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Apa I

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产品名称: Apa I
产品型号: FDRE1124
产品展商: Vivantis Technologies Sdn. Bhd.
美元($)价格: 0.00 元
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发布时间:2020-02-17

简单介绍

Home Restriction Endonucleases Fast Digest Restriction Endonucleases Apa I Apa I Concentration 20u/μl 5 ' . . . G G G C C↓C . . . 3 ' 3 ' . . . C↑C C G G G . . . 5 ' Storage Buffer 10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.


Apa I  的详细介绍
Home  Restriction Endonucleases  Fast Digest Restriction Endonucleases  Apa I

Apa I

Concentration
20u/μl 
5 ' . . . G G G C C↓C . . . 3 '
3 ' . . . C↑C C G G G . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition
20u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of ***** buffer.

Thermal Inactivation
65˚C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay

After 20-fold overdigestion with Apa I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of Apa I for 16 hours at 37˚C.

Ordering Information

Catalog No Description Pack Size
FDRE1124 Apa I 50 preps

 

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Manual

Apa I

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