Ahl I (Spe I)

Concentration
20u/μl
5 ' . . . A C T↓A G T. . . 3 '
3 ' . . . T↑G AT C A . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 1mM DTT,200μg/ml BSA and 50% glycerol.

Unit Definition
20U(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
None

Quality Control Assays
Ligation/ Recutting Assay
After 20-fold overdigestion with Ahl I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40U of Ahl I for 16 hours at 37˚C.

Ordering Information

Downloads
Manual
Ahl I (Spe I)