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ViPrimePLUS Brucella qPCR Kit

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产品名称: ViPrimePLUS Brucella qPCR Kit
产品型号: QM2007
产品展商: Vivantis Technologies Sdn. Bhd.
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简单介绍

Home ViPrimePLUS Real-Time PCR Kits Human Infection qPCR/RT-qPCR Kits Bacterial ViPrimePLUS Brucella qPCR Kit ViPrimePLUS Brucella qPCR Kit Description The ViPrimePLUS Brucella qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of all Brucella species genomes in clinical samples (e.g. blood, milk, body fluids) from animal and human or animal aborted fetuses.


ViPrimePLUS Brucella qPCR Kit  的详细介绍
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ViPrimePLUS Brucella qPCR Kit

Description
The ViPrimePLUS Brucella qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of all Brucella species genomes in clinical samples (e.g. blood, milk, body fluids) from animal and human or animal aborted fetuses.
Brucella spp. are Gram negative bacteria, which cause Brucellosis in both animals and humans. In livestock, they are the main cause of abortion affecting the economy tremendously. There are four different species that affect humans; 1) Brucella melitensis from goats, sheep, and camels is the most prevalent worldwide, 2) Brucella suis from pigs, 3) Brucella abortus from cattle, buffalo, elk, camel and yak, and 4) Brucella canis from dogs. This zoonotic infection spreads from animal to human via contact with or ingestion of infected animals and animal products, especially the unpasteurized milk. Breast-feeding, sexual transmission and organ transplantation are potential methods of person-to-person transmission though the incidences are rare. Generally, brucellosis in human presents a flu-like symptoms and treatment is usually effective with antibiotics. In severe cases, complications involving various organs may follow with ~2% mortality rate. To prevent the potential outbreak, animal vaccination (human vaccine is currently unavailable), animal routine screening and good animal-disease control are important practices.
ViPrimePLUS Brucella qPCR Kit is intended for research use only.

Kit Component

  • Brucella Primers and Probe Mix (Brucella PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit DNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Clinical samples (e.g. blood, milk, body fluids) from animal and human or animal aborted foetuses
Gene Target Phage-related protein gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

Ordering Information

Catalog No Description Pack Size
QM2007 ViPrimePLUS Brucella qPCR Kit 150 reactions

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Manual

ViPrimePLUS Brucella qPCR Kit

 
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