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ViPrimePLUS Rotavirus A RT-qPCR Kit

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产品名称: ViPrimePLUS Rotavirus A RT-qPCR Kit
产品型号: QM5071
产品展商: Vivantis Technologies Sdn. Bhd.
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简单介绍

Home ViPrimePLUS Real-Time PCR Kits Human Infection qPCR/RT-qPCR Kits Virus ViPrimePLUS Rotavirus A RT-qPCR Kit ViPrimePLUS Rotavirus A RT-qPCR Kit Description The ViPrimePLUS Rotavirus A RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Rotavirus A genome in clinical samples (e.g. stool), contaminated food samples and environmental water samples.


ViPrimePLUS Rotavirus A RT-qPCR Kit  的详细介绍
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ViPrimePLUS Rotavirus A RT-qPCR Kit

Description
The ViPrimePLUS Rotavirus A RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Rotavirus A genome in clinical samples (e.g. stool), contaminated food samples and environmental water samples.
Rotavirus is a double-stranded RNA virus that is subdivided into group A to G. Group A, B and C are the most relevant to humans, causing severe acute diarrhoea. Particularly in developing countries, rotavirus gastroenteritis is the major cause of mortality and morbidity among young children. Due to the viral stability in the environment, transmission occurs via ingesting contaminated food and water. The characteristics of the disease are vomiting and watery diarrhoea for 3-8 days, i.e. self-limiting, fever and abdominal pain. Primary infection does not provide complete immunity, however repeat infection tends to be less severe. Vaccination is currently available to cope with the viral infections.
ViPrimePLUS Rotavirus A RT-qPCR Kit is intended for research use only.

Kit Component

  • RotaV A Primers and Probe Mix (RotaV A PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit RNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material • Clinical samples (e.g. stool)
• Food samples
• Environmental water samples
Gene Target Non-structural protein 5 (NSP5) encoding gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included • Internal Extraction Control (IEC) as inhibition control
• Positive control
Channels • FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons

Ordering Information

Catalog No Description Pack Size
QM5071 ViPrimePLUS Rotavirus A RT-qPCR Kit 150 reactions

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Manual

ViPrimePLUS Rotavirus A RT-qPCR Kit

 
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