Concentration
2-10u/μl
5'…CCNNNNN↓NNGG…3'
3'…GGNN↑NNNNNCC…5'
Reaction Conditions
1X Buffer V4
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl2, 100mM KCI, and 100μg/ml BSA. Incubate at 50°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1mM EDTA, 1mM DTT, 200μg/ml BSA and 0.15% Triton X-100, and 50% glycerol. Store at -20°C.
Thermal Inactivation
80°C for 20 minutes.
Ligation / Recutting Assay
After 6-fold overdigestion with Afi I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Afi I for 16 hours at 55°C.
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
Catalog No
|
Description
|
Pack Size
|
RV1116
|
AfiI {BsiYI}
|
500u
|
Downloads
Manual
AfiI {BsiYI}