AluI

Concentration
1-3u/μl
5'…AG↓CT…3' 
3'…TC↑GA…5'

Reaction Conditions 
1X Buffer V5 
30mM Tris-acetate (pH7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 3-fold overdigestion with AluI, 70% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 2u of AluI for 16 hours at 37°C.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

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AluI

Publication
This Product Has Been Used In: 
Badouei, M.A., Jajarmi, M., Mirsalehian, A., (2015) Virulence profiling and genetic relatedness of Shigatoxin-producing Escherichia coli isolated from humans and ruminants. Comparative Immunology, Microbiology and Infectious Diseases. 38. Pp15-20.