Ama87I {AvaI}

Concentration
10-30u/μl
5'…C↓YCGRG…3' 
3'…GRGCY↑C…5'

Reaction Conditions 
1X Buffer Ama87I 
10mM Tris-HCl (pH8.5 at 30°C), 10mM MgCl₂ , 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM KH₂PO₄ (pH 7.2), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with Ama87I, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Ama87I for 16 hours at 37°C.
Supplied with 10X Buffer Ama87I, 10X Buffer UB and Viva Buffer A. (Diluent) 
High enzyme contraction may result in Star Activity.

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Ama87I {AvaI}