BamHI

Concentration
40-150u/μl 
5'…G↓GATCC…3' 
3'…CCTAG↑G…5'

Reaction Conditions 
1X Buffer UB 
25mM Tris-acetate (pH7.6 at 30°C), 10mM Mg-acetate, 100mM K-acetate, 7mM 2-mercaptoethanol, and 50μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton X-100, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 40-fold overdigestion with BamHI, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BamHI for 16 hours at 37°C.
Supplied with 10X Buffer UB and Viva Buffer A. (Diluent) 
*High enzyme concentration may result in Star Activity.

Ordering Information

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Manual
BamHI