Concentration 5u/μl 3 ' . . . C A A↓T T G . . . 5 ' 3 ' . . . C A A↑T T G . . . 5 '
Storage Buffer 10mM Tris-HCl (pH 7.5), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 200μg/ml BSA and 50% glycerol.
Unit Definition 5u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 mins at 37˚C in 20μl or 50μl of assay buffer.
Thermal Inactivation 65˚C for 20 minutes.
Quality Control Assays Ligation/ Recutting Assay After 5-fold overdigestion with Hpa I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Hpa I for 16 hours at 37˚C.
Ordering Information
Downloads Manual Hpa I
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