Sma I

Concentration
20u/μl 
5 ' . . . C C C↓G G G . . . 3 '
3 ' . . . G G G↑C C C . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition
20u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 mins at 25˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65˚C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay
After 20-fold overdigestion with Sma I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of Sma I for 16 hours at 25°C.

Ordering Information

Downloads
Manual
Sma I