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Green fluorescent protein (GFP, EGFP) pAb epitope purified

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产品名称: Green fluorescent protein (GFP, EGFP) pAb epitope purified
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产品展商: Badrilla
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Green fluorescent protein (GFP, EGFP) pAb epitope purified


Green fluorescent protein (GFP, EGFP) pAb epitope purified  的详细介绍
anti-GFP western blot

Green fluorescent protein (GFP, EGFP) pAb epitope purified

$70.00

In stock

Product code: A010-pGFP-5
Unit Size: 5 μg - enough for 31 blots
  • Description
  • Data Sheet

A fully validated, highly specific, affinity purified rabbit polyclonal antibody to GFP, EGFP, PA-GFP, CFP and YFP

Background: The green fluorescent protein (GFP) is composed of 238 amino acid residues (26.9kDa) and exhibits bright green fluorescence when exposed to ultraviolet blue light. It has become a well established reporter for gene expression and marker for protein targeting. Subtle modifications have been made to the sequence of GFP improving its solubility and photostablility (eGFP) to allow acquisition of more complex data (FRET, FRAP). Moreover, subtle residue modifications have been engineered to alter the emission wavelength such as in CFP, YFP to facilitate duel labelling. Anti-GFP Ab is a polyclonal antibody raised in rabbit by immunisation with, and affinity purification against full length GFP (Faix et al., 2001). It produces exceptional staining for GFP, PA-GFP, EGFP, CFP and YFP in western blotting at a dilution of 1:10,000 and bioimaging applications at high dilutions, it has featured in an impressive number of publications.

Tested Applications: Western blot (1:10,000 dilution), IF Microscopy (1:500 - 5000 dilution).

 

Description Lyophilised eabbit polyclonal monoclonal antibody to GFP
Immunogen Recombinant GFP together with complete Freund's adjuvant
Isotype IgG
Purification Affinity Purified using purified GFP covalently coupled to cyanogen bromide activated agarose
Species reactivity No
Tested applications WB, IF, IP, ELISA
Recommended dilution WB 1:10,000, IF 1:500-5000, IP, ELISA
Optimization Optimal dilutions to be determined by end user
Storage Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 6.25 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles.
Storage Lyophilised: 4 °C. Reconstituted:-80 °C
Datasheet A010-pGFP-5

MSDS A010-pGFP-5

Regulatory statement Product for research use only. Not intended for diagnostic or therapeutic use.
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