A fully validated, highly specific affinity purified rabbit polyclonal antibody to Serine-2808 dephosphorylated Ryanodine Receptor

The Ryanodine Receptor (RyR2) is a Ca²⁺ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca²⁺ to ryanodine receptor 2 RyR2 opens the channel and Ca²⁺  stored in the SR moves through the channel into the cytosol to initiate muscle contraction. Abnormal structure and function of ryanodine receptors has been reported in failing hearts, with Ser-2809 phosphorylation appearing elevated in clinical situations which may contribute to the abnormal Ca²⁺  handling characteristics of cardiac muscle in these conditions. Serine-2809 can be phosphorylated by PKA or CaMKII, which is coincident with significant change in RyR2 channel function typified by an increased open probability, the abrogation of the inhibitory effects of CaM and Mg²⁺, dissociation of regulatory factors, expression of subconductance states and the expression of channel activity at diastolic Ca²⁺ concentrations. This antibody recognises Dephosphorylated Ser-2808 (human sequence) or Ser-2809 from rabbit.

Tested applications: Western blot (1:5000 dilution), IHC Microscopy (1:100). Species recognition: all mammalian species, when dephosphorylated on Ser-2808 (or 2809 rabbit).