A fully validated, highly specific rabbit polyclonal antibody to Serine-2814 phosphorylated Ryanodine Receptor 2

The Ryanodine Receptor (RyR2) is a Ca²⁺ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca²⁺ to Ryanodine Receptor 2 RyR2 opens the channel and Ca²⁺ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. CaMKII, was able to phosphorylate Ser-2814 of RYR2 enhancing Ca²⁺- sensitivity and increasing open probability. Excessive phosphorylation of Ser-2814 leads to atrial fibrillation, arrhythmias and sudden death

Tested applications: Western blot (1:5000 dilution), immunofluorescence microscopy (1:100). Species recognition: All mammalian species, when phosphorylated on Ser-2814 (or Ser-2815 rabbit) Antibody recognises epitope despite single residue difference in epitope between species (V2815I; human vs mouse).