A fully validated, highly specific rabbit polyclonal antibody to Ryanodine Receptor 2 phospho Serine-2808

The Ryanodine Receptor (RyR2) is a Ca²⁺ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca²⁺  to ryanodine receptor 2 (RyR2) opens the channel and Ca²⁺ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. Abnormal structure and function of ryanodine receptors has been reported in failing hearts, with Ser-2808 phosphorylation appearing elevated in clinical situations which may contribute to the abnormal Ca²⁺ handling characteristics of cardiac muscle in these conditions. Ser-2808 can be phosphorylated in vitro by PKA or CaMKII, which is coincident with significant change in ryanodine receptor 2 RyR2 channel function typified by an increased open probability, the abrogation of the inhibitory effects of CaM, and Mg²⁺, dissociation of regulatory factors, expression of subconductance states and the expression of channel activity at diastolic Ca²⁺ concentrations. Serine-2808 in human RyR2 equates to Ser-2809 in rabbit RyR2.

Tested applications: Western blot (1:5000 dilution), IHC Microscopy (1:100 dilution). Species recognition: human, rat, mouse, rabbit, dog, canine and sheep.