Affinity purified polyclonal antibody (IgG) to Serine-16 phosphorylated forms of phospholamban from the original polyclonal antiserum of Drago & Colyer (1994)

Phospholamban (PLB/PLN) is a small transmembrane protein which plays an important role in controlling the activity of the sarcoplasmic reticulum ATPase (SERCA2a) of cardiac muscle during calcium sequestration. Phospholamban is phosphorylated on separate amino acid residues (Ser-16, Thr-17, Ser-10) by different kinases. cAMP-dependent, and cGMP-dependent protein kinases phosphorylate Ser-16. The result is an increased calcium pump activity which reduces the time course of the calcium transient, increases the calcium load in the sarcoplasmic reticulum, and consequently, produces a larger calcium transient at the next action potential. However, alteration in this homeostatic interaction has been shown to result in heart failure, underpin arrhythmias and provokes a change in phenotype of smooth muscle cells in atherosclerosis.

Tested applications: Western Blot (1:5000 dilution), IHC Microscopy (1:200 dilution). Not yet tested in other applications, therefore, optimal dilutions/concentrations should be determined by the user. Species recognition: All mammalian species, when phosphorylated on Ser-16.