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FAM amine, 6-isomer

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产品名称: FAM amine, 6-isomer
产品型号: 151C0 251C0 451C0 551C0 651C0
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FAM amine, 6-isomer 151C0 251C0 451C0 551C0 651C0


FAM amine, 6-isomer  的详细介绍

FAM amine, 6-isomer

Fluorescein (FAM) Amines
Cat. # Quantity Price Lead time
151C0 1 mg  $110.00 in stock
 
251C0 5 mg  $210.00 in stock
 
451C0 25 mg  $410.00 in stock
 
551C0 50 mg  $695.00 in stock
 
651C0 100 mg  $1190.00 in stock
 
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Fluorescein derivative with amine group, contains pure 6-isomer of the fluorophore. This reagent can be used for the modification of biomolecules by enzymatic transamination. Its aliphatic amine groups also reacts with electrophiles (like activated esters). This amine can be also conjugated with carbonyl compounds (aldehydes and ketones) by means of reductive amination.

Structure of 6-FAM amine
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Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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FAM amine, 5-isomer

Amino derivative of fluorescein, pure 5-isomer. Contains a linker arm between the aliphatic amine group, and the fluorophore. Useful for the labeling via enzymatic transamination, for the reactions with electrophilic reagents, and for reductive amination.
 

TAMRA amine, 5-isomer

TAMRA (tetramethylrhodamine) dye, pure 5-isomer, with an amine group for the conjugation with electrophilic reagents (NHS esters, epoxides, etc).
 
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General properties

Appearance: yellow solid
Molecular weight: 510.97
Molecular formula: C27H27N2ClO6
IUPAC name: 5-aminohexylaminocarbonylfluorescein hydrochloride
Solubility: good in methanol, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 490
ε, L?mol?1?cm?1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17
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