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dsGreen for Real-Time PCR, 100×

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产品名称: dsGreen for Real-Time PCR, 100×
产品型号: 41010
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dsGreen for Real-Time PCR, 100× 41010


dsGreen for Real-Time PCR, 100×  的详细介绍

dsGreen for Real-Time PCR, 100×

PCR and qPCR
Cat. # Quantity Price Lead time
41010 1 mL  310.00$ in stock
 
Bulk inquiry 

dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.

Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:

  • Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
  • PCR tested preparation – quality guaranteed
  • Low fluorescence background – high fluorescence intensity gain

Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

dsGreen for Real-Time PCR, 100×
dsGreen for Real-Time PCR, 100×
dsGreen for Real-Time PCR, 100×
dsGreen structure
Download in 
ChemDraw format

qPCR curves with dsGreen

qPCR curves with dsGreen

Recommended protocol

qPCR with dsGreen or SYBR Green I

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General properties

Appearance: light orange solution
Quality control: NMR 1H, HPLC-MS (95%), PCR testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 454
ε, L?mol?1?cm?1: 73000
Emission maximum, nm: 524
Fluorescence quantum yield: 0,8

Product citations

  1. Schmidt, N.; Kollewe, A.; Constantin, C.E.; Henrich, S.; Ritzau-Jost, A.; Bildl, W.; Saalbach, A.; Hallermann, S.; Kulik, A.; Fakler, B.; Schulte, U. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron, 2017, 96(4), 827–838.e9. doi: 10.1016/j.neuron.2017.09.038
  2. Revalde, J.L.; Li, Y.; Wijeratne, T.S.; Bugde, P.; Hawkins, B.C.; Rosengren, R.J.; Paxton, J.W. Curcumin and its cyclohexanone analogue inhibited human Equilibrative nucleoside transporter 1 (ENT1) in pancreatic cancer cells. European Journal of Pharmacology, 2017, 803, 167–173. doi: 10.1016/j.ejphar.2017.03.055
  3. Wu, Z.-G.; Jiang, W.; Chen, S.-L.; Mantri, N.; Tao, Z.-M.; Jiang, C.-X. Insights from the Cold Transcriptome and Metabolome ofDendrobium officinale: Global Reprogramming of Metabolic and Gene Regulation Networks during Cold Acclimation. Frontiers in Plant Science, 2016, 7, 1653. doi: 10.3389/fpls.2016.01653
  4. Brauer, V.S.; Stomp, M.; Bouvier, T.; Fouilland, E.; Leboulanger, C.; Confurius-Guns, V.; Weissing, F.J.; Stal, L.J.; Huisman, J.Competition and facilitation between the marine nitrogen-fixing cyanobacterium Cyanothece and its associated bacterial community.Frontiers in Microbiology, 2015, 5, 795. doi: 10.3389/fmicb.2014.00795
SYBR® is a registered trademark of Molecular Probes.
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