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ROX reference dye for qPCR

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产品名称: ROX reference dye for qPCR
产品型号: 31110 41110
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ROX reference dye for qPCR 31110 41110


ROX reference dye for qPCR  的详细介绍

ROX reference dye for qPCR

ROX (Rhodamine X, Rhodamine 101) PCR and qPCR
Cat. # Quantity Price Lead time
31110 500 uL  22.00$ in stock
 
41110 1 mL  30.00$ in stock
 
Bulk inquiry 

This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as SYBR Green I or TaqMan probes. It also allows to correct for pipeting errors, and instrumental drift such as change of lamp intensity output over time.

The dye contains 25 μM solution of 5-carboxy-ROX (rhodamine-X) reference dye in 10mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween®-20.

ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.

ROX reference dye for qPCR
ROX reference dye for qPCR
ROX reference dye for qPCR

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General properties

Appearance: pink solution
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 570
Emission maximum, nm: 591

Product citations

  1. Schmidt, N.; Kollewe, A.; Constantin, C.E.; Henrich, S.; Ritzau-Jost, A.; Bildl, W.; Saalbach, A.; Hallermann, S.; Kulik, A.; Fakler, B.; Schulte, U. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron, 2017, 96(4), 827–838.e9. doi: 10.1016/j.neuron.2017.09.038
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