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Azidobutyric acid NHS ester

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产品名称: Azidobutyric acid NHS ester
产品型号: 33720 43720 53720 63720
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简单介绍

Azidobutyric acid NHS ester 33720 43720 53720 63720


Azidobutyric acid NHS ester  的详细介绍

Azidobutyric acid NHS ester

NHS esters Protein labeling
Cat. # Quantity Price Lead time
33720 10 mg  60.00$ in stock
 
43720 25 mg  95.00$ in stock
 
53720 50 mg  185.00$ in stock
 
63720 100 mg  290.00$ in stock
 
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Convert your proteins and peptides into Click Chemistry reactive form with this reagent.

While Click Chemistry involves reaction between terminal alkyne and azide, both azides and alkynes are very uncommon in nature. However, there are reagents to attach these fragments to abundant amino groups which are ubiquitous in the world of biomolecules. This azido-NHS ester is designed for the conversion of proteins, peptides, amino-DNA, and other amines into Click Chemistry reactive azides.

Azidobutyric acid NHS ester
Azidobutyric acid NHS ester
Azidobutyric acid NHS ester
Structure of azidobutyric acid NHS ester
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Recommended protocol

NHS ester labeling of amino biomolecules

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Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.
 

Alkyne hydrazide

Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.
 

Alkyne maleimide

Maleimide reagent for the attachment of alkyne functional group to proteins, peptides, and other molecules bearing SH (thiol) groups, including cysteine residues.
 

General properties

Appearance: colorless solid
Mass spec M+ increment: 111.0
Molecular weight: 226.19
Molecular formula: C8H10N4O4
Solubility: soluble in organic solvents (DMF, DMSO)
Quality control: NMR 1H (95%), HPLC
Storage conditions: Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download

Product citations

  1. Taskova, M.; Uhd, J.; Miotke, L.; Kubit, M.; Bell, J.; Ji, H.P.; Astakhova, K. Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence. Analytical Chemistry, 2017, 89(8), 4363–4366. doi: 10.1021/acs.analchem.7b00646
  2. Bu, J.; Pilo, A.L.; McLuckey, S.A. Gas Phase Click Chemistry via Ion/Ion Reactions. International Journal of Mass Spectrometry, 2015, 390, 118–123. doi: 10.1016/j.ijms.2015.05.010
  3. Potapova, I.; Eglin, D.; Laschke, M.W.; Bischoff, M.; Richards, R.G.; Moriarty, T.F. Two-step labeling of Staphylococcus aureus with Lysostaphin-Azide and DIBO-Alexa using click chemistry. Journal of Microbiological Methods, 2013, 92(1), 90-98. doi:10.1016/j.mimet.2012.11.004
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