主营产品:

小鼠肺细胞,仓鼠体细胞,小鼠神经细胞,小鼠脑细胞,小鼠淋巴细胞,仓鼠细胞,兔肝细胞,兔上皮细胞,兔胰岛细胞,猪上皮细胞

产品详情
如果您对该产品感兴趣的话,可以
产品名称: iN-LSC
产品型号: S10004
产品展商: 其它品牌
美元($)价格: 89.00 元
欧元(€)价格: 0.00 元
人民币(¥)价格: 0.00 元
产品文档: 无相关文档
发布时间:2019-12-06

简单介绍

iN-LSC


iN-LSC  的详细介绍
Product Information
Purity: ≥98% for each compound
Storage: 4oC for 3 month. -20oC for 6 month.
Cocktail Formulation: 0.5 mM LDN193189, 10 mM SB431542, 2 mM CHIR99021 in DMSO solution
Effective Concentration in Cell Culture: 0.5 µM LDN193189, 10 µM SB431542, 2 µM CHIR99021 in cell culture medium

Biological Activity:

In previous studies, dual SMAD inhibition using small molecule inhibitors of BMP and TGFβ pathways, i.e., LDN193189 and SB431542 respectively, has been shown to induce efficient neural differentiation of human pluripotent stem cells. In a recent publication, it was shown that combination of LDN193189/SB431542 with a specific GSK-3β inhibitor (CHIR99021) could significantly promote the conversion of postnatal human fibroblasts transduced with Ascl1 and Ngn2 into functional neuron-like cells with yields up to 200% and neuronal purities up to 80%.

How to Use:

  • In vitro: Dilute received Ready-to-use iN-LSC stock solution at 1:1000 into your neuronal reprograming medium to make the neuron induction media and use it to initiate neuronal reprogramming.

Reference:

  1. 1.  Ladewig J, et al. Small molecules enable highly efficient neuronal conversion of human fibroblasts. (2012) Nature Methods 9(6), p575-578.

  • Product Specification:
  • Product MSDS:



Products are for research use only. Not for human use.

产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
Copyright@ 2003-2019  上海斯信生物科技有限公司  版权所有  

本公司网站所展示销售的产品仅供科研!

         沪ICP备17033327号-1   

沪公网安备 31011202007337号