ELISA
96 wells/40 (in duplicate)
Plasma and serum
96 well assay plate
Reactive with human derived TRAP5b only. No reactivity detected with Cow, Chicken, Goat, Mouse, Rabbit, Rat, Sheep, Guinea Pig, Baboon, Cynomolgus Monkey, Rhesus Macaque
Add 100 µL of Sample Diluent Add 50 µL of Calibrator, Controls and Specimens Seal the microplate Incubate for 60 minutes at room temperature on a microplate shaker Wash the microplate wells three times with Wash Buffer Add 100 µL of Substrate Solution Seal the microplate Incubate for 60 minutes at 37°C Add 50 µL of Stop Solution Read OD at 405 nm Data Reduction Quadratic
ELISA Kit
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