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MicroVue Serum PYD EIA* Pyridinoline crosslinks (serum)[产品打印页面]

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产品名称: MicroVue Serum PYD EIA* Pyridinoline crosslinks (serum)
产品型号: 8019
产品展商: Quidel
美元($)价格: 870.00 元
欧元(€)价格: 0.00 元
人民币(¥)价格: 0.00 元
产品文档: 无相关文档
发布时间:2019-08-21

简单介绍

MicroVue Serum PYD EIA* Pyridinoline crosslinks (serum)


MicroVue Serum PYD EIA* Pyridinoline crosslinks (serum)  的详细介绍

Features & Benefits

Feature Benefit

Product Specifications

Product Spec Name Product Spec Data
Format

ELISA

Test/kit

96 wells/plate

Sample type

Serum

Performance Characteristics

Method: Competitive
Analyte: Pyridinoline
Specimen: Serum
Specimen Volume: 25 µL post-filtration
LOD*: 0.4 nmol/L
LLOQ*: 0.75 nmol/L
HLOQ*: 12 nmol/L
Precision CVs within-run: 6-15%
Precision CVs between-run: 9-12%
Incubation Time: 20-26 Hours
Cross-reactivity: Free PYD 100% Free DPD 0% PYD/DPD peptides < 2.5% L-arginine 0.0004% (see Package Insert)

*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

About the Assay

Format

96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate

Antibody Specificity

Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)

Species Reactivity

Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat
No reactivity detected with Rabbit

Assay Steps

Spin serum in Spinfilters (included) for 30 minutes
Add 50 µL Reagent 1 to plate
Add 25 µL serum, diluted Standards, diluted Controls
Add 75 µL cold Pyridinoline Antibody
Incubate overnight at 2°C to 8°C
Wash 3 times with 1x Wash Buffer
Add 150 µL Enzyme Conjugate
Incubate 1 hour at room temperature
Wash 3 times with 1x Wash Buffer
Add 150 µL working Substrate Solution
Incubate 40 +/– 5 minutes at room temperature
Add 100 µL Stop Solution
Read at 405 nm
Data Reduction: 4 Parameter

Ordering Information

Catalog Number Description
8019

96 Wells/Plate

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