For Research Use Only in the United States. Not for use in diagnostic procedures.
The MicroVue CICP assay measures type I C-terminal collagen propeptide (CICP) in serum. CICP may be useful as a biochemical indicator of collagen production and growth.
Collagen, the triple-helical molecule which forms the fibrous framework of all connective tissues, is synthesized as procollagen, a larger precursor molecule. Procollagen consists of mature collagen with extension peptides at both the amino and carboxy termini. These extension peptides, or propeptides, are cleaved from the collagen molecule by specific proteases prior to incorporation of collagen into a growing collagen fibril. The release of these peptides into the circulation provides a stoichiometric representation of the production of collagen.
ELISA
96 wells/plate
Serum
Method: Sandwich Analyte: Collagen type 1 C-terminal propeptide Specimen: Serum Specimen Volume: 25 µL LOD*: 0.2 ng/mL LLOQ*: 1 ng/mL HLOQ*: 80 ng/mL Precision CVs within-run: 5%-7% Precision CVs between-run: 5%-7% Incubation Time: 4 Hours Cross-reactivity: CICP 100%
*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
96-well microplate with reagents sufficient to test 40 samples in duplicate.
Rabbit, Cynomolgus Monkey, Sheep, Human Cell Culture No Recognition of Rat, Dog, Goat, or Horse
Add 100 µL Standards, Controls and 1:12 diluted samples Incubate 120 ± 5 minutes at 18°C to 25°C Wash 3 times with 1X Wash Buffer Add 100 µL Rabbit anti-CICP Incubate 45-50 minutes at 18°C to 25°C Wash 3 times with 1X Wash Buffer Add 100 µL Enzyme Conjugate Incubate 45-50 minutes at 18°C to 25°C Wash 3 times with 1X Wash Buffer Add 100 µL Working Substrate Solution Incubate 30-35 minutes at 18°C to 25°C Add 50 µL Stop Solution and read Optical Density at 405 nm Data Reduction: 4 Parameter
ELISA Kit
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