Western blot of nonreduced(-) and reduced(+)mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mLof RevMAb clone RM103. This antibody reacts to nonreduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).
ELISA of mouse immunoglobulins shows RM103reacts to the kappa light chain of mouse immuno-globulins. No cross reactivity with the lamda lightchain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno-globulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL ofRM103 was used as the primary antibody. Analkaline phosphatase conjugated anti-rabbitIgG as the secondary antibody.
A titer ELISA of mouse IgG1κ. The plate was coatedwith different amounts of mouse IgG1κ. A serial dilution of RM103 was used as the primary antibody.An alkaline phosphatase conjugated anti-rabbitIgG as the secondary antibody.
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