Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
ELISA of human immunoglobulins shows RM117 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
本公司网站所展示销售的产品仅供科研!
沪公网安备 31011202007337号