RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3.
Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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