For Research Use Only. Not for use in diagnostic procedures.
The MicroVue Helical Peptide is a test to measure type I collagen helical peptide α1(I) 620-633 in urine, a marker for researching collagen degradation.
Type I collagen is degraded during bone resorption, releasing into circulation peptides of various molecular weights that are further degraded and/or released into urine. A peptide consisting of residues 620-633 derived from the helical region of the α1 chain was isolated from the urine of a patient with Paget's disease.
ELISA
96 wells/plate
Urine
Method: Competitive Analyte: Helical Peptide α1(I) 620-633 Specimen: Urine Specimen Volume: 20 µL LOD*: 8 µg/L LLOQ*: 20.2 µg/L HLOQ*: 2514 µg/L Precision CVs within-run: 4%-8% Precision CVs between-run: 7%-17% Incubation Time: 19-25 Hours Cross-reactivity: Helical peptide α1(I) 620-633 100% Intact collagen I, II, III, IV < 1% Homologous peptides (see Package Insert)
*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
96-well microplate with reagents sufficient to test 40 samples in duplicate
Helical peptide α1(I) 620-633; no reactivity with intact collagen types I, II, III, IV or mixtures of amino acids; 90% reactivity with α1(III) homologous peptide; no reactivity with α1(I) or α2(II) homologous peptides
Rat, Mouse, Rabbit, Guinea Pig, Cat, Dog, Pig, Goat, Sheep, Cow, Horse, Rhesus Macaque, Cynomolgus Monkey, Baboon, Chimpanzee, Human Cell Culture
Add 20 µL Standards, Controls, and samples Add 150 µL Enzyme Conjugate
ELISA Kit
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