For In Vitro Diagnostic Use.
MicroVue PYD provides a quantitative measure of pyridinium cross-links (Pyd and Dpd) as an indicator of type I collagen resorption, especially bone collagen. Pyd and Dpd are excreted unmetabolized in urine.
MicroVue PYD is intended for use as an indicator of type I collagen resorption, especially bone collagen.
ELISA
96 wells/plate
Urine
Method: Competitive Analyte: Pyridinium cross-links Specimen: Urine Specimen Volume: 50 µL Sensitivity: 7.5 nM/L Dynamic Range: 15-750 nM Precision CVs within-run: 6%-10% Precision CVs between-run: 3%-11% Incubation Time: 4 Hours Cross-reactivity: Free Dpd 100% Free Pyd 100% Pyd/Dpd peptides < 2.5% Expected Values: Females: >25 yr. premenopausal: 16.0-37.0 nmol Pyd/mmol Cr Males: 12.8-25.6 nmol Pyd/mmol Cr
96-well microplate with reagents sufficient to test 40 samples in duplicate Antibody Specificity: The antibody in the assay has a selective, high affinity for free Pyd and Dpd and negligible binding to Pyd and Dpd peptides.
Mouse, Rat, Guinea Pig, Rabbit, Pig, Dog, Sheep, Horse, Cynomolgus Monkey, Rhesus Macaque, Human Cell Culture
96 Wells/Plate
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