ELISA
96 wells/plate
Serum
Method: Competitive Analyte: Pyridinoline Specimen: Serum Specimen Volume: 25 µL post-filtration LOD*: 0.4 nmol/L LLOQ*: 0.75 nmol/L HLOQ*: 12 nmol/L Precision CVs within-run: 6-15% Precision CVs between-run: 9-12% Incubation Time: 20-26 Hours Cross-reactivity: Free PYD 100% Free DPD 0% PYD/DPD peptides < 2.5% L-arginine 0.0004% (see Package Insert)
*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate
Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)
Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat No reactivity detected with Rabbit
Spin serum in Spinfilters (included) for 30 minutes Add 50 µL Reagent 1 to plate Add 25 µL serum, diluted Standards, diluted Controls Add 75 µL cold Pyridinoline Antibody Incubate overnight at 2°C to 8°C Wash 3 times with 1x Wash Buffer Add 150 µL Enzyme Conjugate Incubate 1 hour at room temperature Wash 3 times with 1x Wash Buffer Add 150 µL working Substrate Solution Incubate 40 +/– 5 minutes at room temperature Add 100 µL Stop Solution Read at 405 nm Data Reduction: 4 Parameter
96 Wells/Plate
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