ELISA of mouse immunoglobulins shows RM218 reacts to both mouse IgG3κ and IgG3λ; No crossreactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coatedwith 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 wasused as the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgG3. The plate was coatedwith different amounts of mouse IgG3. A serialdilution of RM218 was used as the primary antibody.An alkaline phosphatase conjugated anti-rabbit IgGas the secondary antibody.
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