ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coatedwith 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was usedas the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondaryantibody.
A titer ELISA of mouse IgG2a. The plate wascoated with different amounts of mouse IgG2a. A serial dilution of RM219 was used as the primaryantibody. An alkaline phosphatase conjugatedanti-rabbit IgG as the secondary antibody.
Western blot of nonreduced(-) and reduced(+)mouse IgG2a, using 0.5ug/mL of RevMAb cloneRM219. This antibody reacts to nonreducedIgG2a (~150 kDa).
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