Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146

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产品名称: Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146

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Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146

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Product Details
Product Name	Biotin Anti-His-Tag (6xHis-Tag or 10xHis-Tag) Rabbit Monoclonal Antibody [RM146]
Product Description	Biotinylated Rabbit monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
Catalog No.	31-1048-02
Clone Name	RM146
Product Data Sheet	Biotin Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146
Specificity	RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Immunogen	Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Application	Western Blot ELISA Immunoprecipitation Immunocytochemistry Flow Cytometry Immunohistochemistry
Species Reactivity	All
Conjugate	Biotin
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.1 ug/mL – 0.5 ug/mL; Immunocytochemistry 0.5 ug/mL – 2 ug/mL; Flow Cytometry: 0.5 ug/mL – 2 ug/mL; Immunohistochemistry; 0.1 ug/mL-1 ug/mL; ELISA: 0.01 ug/mL-0.5 ug/mL.
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Product Images
	A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of Biotin Anti-HisTag Rabbit Monoclonal Antibody Clone RM146 was used as the primary antibody, followed byalkaline phosphatase conjugated Streptavidin.
	Western blot of 293T cells transfected (+) or untrans-fected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using Biotin Anti-HisTag Rabbit Monoclonal Antibody Clone RM146 at 0.2 ug/mL,followed by a HRP conjugated Streptavidin.
	Immunohistochemistry staining of naïve HepG2 cells(Negative control) using anti-His-Tag antibody, RM146.
	Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using anti-His-Tag antibody, RM146.

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