Anti-Phosphotyrosine Rabbit Monoclonal Antibody, Clone RM111

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产品名称: Anti-Phosphotyrosine Rabbit Monoclonal Antibody, Clone RM111

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Anti-Phosphotyrosine Rabbit Monoclonal Antibody, Clone RM111

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Product Details
Product Name	Anti-Phosphotyrosine Rabbit Monoclonal Antibody [RM111]
Product Description	Rabbit monoclonal to Phosphotyrosine
Catalog No.	32-1011-00
Clone Name	RM111
Product Data Sheet	Anti-Phosphotyrosine Rabbit Monoclonal Antibody Data Sheet RM111
Specificity	RM111 reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine
Immunogen	Phosphotyrosine-BSA conjugate
Application	Western Blot (WB) Immunoprecipitation (IP) Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Species Reactivity	All Species
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size	100ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot (WB): 1:1000 -1:5000 dilution; Immunoprecipitation (IP): 1:200-1:1000 dilution; Chromatin IP (ChIP): 1:200-1:1000 dilution; Immunocytochemistry (ICC): 1:200-1:500 dilution; Immunohistochemistry (IHC): 1:200-1:500 dilution.
Keywords
References for Phosphotyrosine Rabbit Monoclonal Antibody [RM111]	Sun et al., Neuregulin-1/ErbB4 Signaling Regulates Visual Cortical Plasticity, Neuron (2016), http://dx.doi.org/ 10.1016/j.neuron.2016.08.033. IHC; Mouse Read more (PubMed: 27641496)
Product Images
	Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using RevMAb Clone RM111 at 1/500 dilution (foll-owed by a PE conjugated secondary antibody,red) and DAPI (blue).
	Western blot of serum-starved A431 cells non-treated or treated with EGF, using RevMAbClone RM111 at 1/5000 dilution. The blot was exposed to the film at different time points.
	Immunoprecipitation of EGF-treated A431 cells by RevMAb Cone RM111 at 1/1000 dilution, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by Clone RM111.
	Luminex analysis of Clone RM111’s reaction against phosphorylated or nonphosphorylatedSerine, Threonine, and Tyrosine. RevMAb Clone RM111 reacts only to phosphorylated Tyrosine.

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