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Detection of human IgG3 in monkey serum. Sandwich ELISA using RM119 as the capture antibody, and biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.
Sandwich ELISA using RM119 as the capture antibody (100ng/well), and biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an HRP conjugated streptavidin.
ELISA showing RM119 reacts only to human IgG3, and not to any other IgG subclasses (IgG1, IgG2, or IgG4), and no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM119 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG3 rabbit monoclonal antibody (Clone RM119).
ELISA showing RM119 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM119 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG3 antibody RM119 at a 1:2000 dilution.
Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA
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