Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
Sandwich ELISA using RM117 as the capture antibody, and biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
ELISA showing RM117 reacts only to human IgG1, and not to any other IgG subclasses (IgG2, IgG3, or IgG4), and no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA showing RM117 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM117 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG1 antibody RM117 at a 1:15,000 dilution.
Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA
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