Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody, Clone RM199

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产品名称: Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody, Clone RM199

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Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody, Clone RM199

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Product Details
Product Name	Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody [RM199]
Product Description	Rabbit monoclonal to Acetylated Histone H4 Lysine 5 (K5ac), H4K5ac, Histone H4 (K5 acetylation)
Catalog No.	31-1081-00
Clone Name	RM199
Product Data Sheet	Anti-Acetyl-Histone H4 Lys5 Rabbit Monoclonal Antibody Data Sheet RM199
Specificity	RM199 reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with other acetylated Lysines in Histone H4.
Immunogen	An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys5).
Application	Western Blot (WB) ELISA Multiplex Immunocytochemistry (ICC)
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.2 ug/mL - 1 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.05 ug/mL – 0.2 ug/mL; Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
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Product Images
	RM199 specifically reacts to Histone H4 acetylatedat Lysine 5 (K5ac). No cross reactivity with unmodifiedLysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac),Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
	Western Blot of (1) acid extracts of HeLa cells; (2)recombinant Histone H4. Using RM199 at 0.2 ug/mL,showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.
	Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody (clone RM199) (red). Actin filaments have been labeled with fluorescein phalloidin (green).

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