RM208 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No crossreactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.
Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM208 at 1 ug/mL,showed a band of Histone H4 trimethylated at Lysine20 in HeLa cells.
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