Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody, Clone RM195

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产品名称: Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody, Clone RM195

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Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody, Clone RM195

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Product Details
Product Name	Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody [RM195]
Product Description	Rabbit monoclonal to Monomethylated Histone H4 Arginine 3, H4R3me1, Histone H4 (monomethyl R3)
Catalog No.	31-1080-00
Clone Name	RM195
Product Data Sheet	Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody Data Sheet RM195
Specificity	RM195 reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
Immunogen	A monomethyl -peptide corresponding to the Monomethyl -Histone H4 (Arg3).
Application	Western Blot (WB) ELISA Multiplex Immunocytochemistry (ICC)
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.2 ug/mL – 1 ug/mL; Immunocytochemistry: 1 ug/mL- 2 ug/mL.
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Product Images
	RM195 specifically reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylatedArg 3 (R3me2a) or symmetric dimethylated Arg 3(R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.
	Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM195 at 0.5 ug/mL,showed a band of Histone monomethylated at Arginine3 in HeLa cells.
	Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody (clone RM195) (red). Actin filaments have been labeled with fluorescein phalloidin (green).

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