Western Blot of (1) acid extracts of HeLa cells; (2)recombinant Histone H4. Using RM194 at 0.5 ug/mL,showed a band of Histone H4 phosphorylated atSerine 1 in HeLa cells.
RM194 specifically reacts to Histone H4 phosphorylatedat Serine 1 (H4S1p). The reactivity is not affected byneighboring Arginine 3 modifications (H4S1pR3me1and H4S1pR3me2). No cross reactivity with Histone H2Aphosphorylated at Serine 1 (H2AS1p).
Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H4 (Ser1) Rabbit mAb RM194 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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