Anti-Histone H3 Pan Rabbit Monoclonal Antibody, Clone RM188

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产品名称: Anti-Histone H3 Pan Rabbit Monoclonal Antibody, Clone RM188

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Anti-Histone H3 Pan Rabbit Monoclonal Antibody, Clone RM188

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Product Details
Product Name	Anti-Histone H3 pan Rabbit Monoclonal Antibody [RM188]
Product Description	Rabbit monoclonal to Histone H3 pan
Catalog No.	31-1057-00
Clone Name	RM188
Product Data Sheet	Anti-Histone H3 pan Rabbit Monoclonal Antibody Data Sheet RM188
Specificity	RM188 reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins
Immunogen	A peptide corresponding to the C-terminus of human Histone H3.
Application	Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.01 ug/mL – 0.25 ug/mL; ICC: 1 ug/mL - 5 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
	RM188 reacts to both Histone H3.1 and Histone H3.3.
	Western Blot of recombinant Histone H3.3 andHistone H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using RM188at 0.025 ug/mL, showed a band of recombinant H3.1 and H3.3 proteins, and total histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.
	Immunocytochemistry of HeLa cells, using Histone H3 Rabbit mAb RM188 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
	Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161,1 ug/mL) as the detection antibody.
	Sandwich ELISA against acetylated histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3K79ac (RM156, 5 ug/mL) as the capture antibody and biotinylated anti-H3CT (RM188, 1 ug/mL) as the detection antibody.

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