RM147 specifically reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated Lysine 79 (K79me2), trimethylated Lysine 79 (K79me3), or other methylations in histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM147 at 0.5 ug/mL, showed a band of histone H3 mono-methylated at Lysine 79 (K79me1) in HeLa cells.
ChIP performed on HeLa cells using H3K79me1 antibody (RM147, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
本公司网站所展示销售的产品仅供科研!
沪公网安备 31011202007337号