RM180 specifically reacts to Histone H3 monomethy- lated at Lysine 56 (K56me1). No cross reactivity with nonmodified Lysine 56 (K56Ctrl), or other methylationsin histone H3.
Western Blot of acid extracts of HeLa cells (1) andrecombinant histone H3.3 (2), using RM180 at 1 ug/mL, showed a band of histone H3 monomethylated atLysine 56 (K56me1) in HeLa cells.
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