RM141 specifically reacts to Histone H3 dimethylatedat Lysine 36 (K36me2). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1), trimethylated Lysine 36 (K36me3), or other methylations in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM141 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 36 (K36me2) in HeLa cells.
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