A Peptide dotblot shows RM171 only reacts to Histone. H3 dimethyl-Lysine 23 (K23me2). No cross reactivity. with nonmodified Lysine 23 (K23Ctrl), monomethylated. Lysine 23 (K23me1), or dimethylated Lysine 4 (K4me2).
RM171 specifically reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with other methylated lysines in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM171 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 23 (K23me2) in HeLa cells.
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