A Peptide dotblot shows RM168 only reacts to Histone. H3 dimethyl-Lysine 18 (K18me2). No cross reactivity. with nonmodified Lysine 18 (K18Ctrl), monomethylated. Lysine 18 (K18me1), dimethylated Lysine 4 (K4me2),. or dimethylated Lysine 36 (K36me2).
RM168 specifically reacts to Histone H3 dimethylated at Lysine 18 (K18me2). No cross reactivity with other methylated lysines in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM168 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 18 (K18me2) in HeLa cells.
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