Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151

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产品名称: Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151

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Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151

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Product Details
Product Name	Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM151]
Product Description	Rabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2, Histone H3 (dimethyl K9)
Catalog No.	31-1059-00
Clone Name	RM151
Product Data Sheet	Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM151
Specificity	RM151 reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in histone H3.
Immunogen	A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9).
Application	Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.25 ug/mL – 1 ug/mL; ChIP: 2 ug/mL- 10 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
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Product Images
	RM151 specifically reacts to Histone H3 dimethylatedat Lysine 9 (K9me2). No cross reactivity with non-modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3.
	Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM151 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 9 (K9me2) in HeLa cells.
	ChIP performed on HeLa cells using H3K9me2 antibody (RM151, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
	Immunocytochemistry of HeLa cells treated withsodium butyrate, using Dimethyl-Histone H3(Lys9) Rabbit mAb RM151 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).

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